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. 2013 Sep 17;8(9):e74862. doi: 10.1371/journal.pone.0074862

Figure 4. Effect of probenecid on accumulation and retention of calcein (Cal) in L. donovani isolates.

Figure 4

A. Effect of probenecid on accumulation of Cal: accumulation under normal condition (hatched bar), accumulation under ATP depletion (mat bar), de-energized Cal accumulation in presence of probenecid (white dotted bar). B. Effect of probenecid on Cal efflux: Cal retention after 1 h 30 min efflux (hatched bar), retention of Cal in presence of probenecid at time of accumulation (mat bar), retention in presence of probenecid at time of efflux (white dotted bar). Results are mean of three independent experiments performed from three different promastigote cultures. * P ≤ 0.05, ** P ≤ 0.005 indicate statistical significance with respect to sensitive isolate S1 for resistant isolate R5 and reference sensitive strain Dd8 for lab resistant mutant Mt, ns indicates no statistically significant difference.