Table 1. Chosen internal control and target genes; primer design and PCR conditions.

Gene	Protein	Sequence of forward and reverse primers	Final primer conc.(nM)	Annealing temperature (°C)
ATUB	Alpha-tubulin	5’AGGATGCGGCGAACAACTAC3’	300	61
		5’CAGCGTGGAACACCATAAAGC3’
MRPA	ABC transporter	5’CGCAGCCGTTTGTGCTTGTGG3’	300	55
		5’ TGCCGTACGTCGCGATGGTGC3’
AQP1	Aquaglyceroporin1	5’ TGGCGAACTGTGTCTTTGGT3’	500	46
		5’GTACTTGGACGCCATCGT3’
γ-GCS	γ-Glutamylcysteine synthetase	5’ACATTGGCTGGCGCGTTGAGT3’	500	46
		5’GACGATGGAGATCTTGGTGTA3’
ODC	Ornithine decarboxylase	5’ CAGCGGCAACGACGACCAGT3’	500	61
		5’GTGACATCACCGCCGCCGAA3’
TR	Trypanothione reductase	5’ GGCGAGGTTCTGGGTGTTC3’	300	58
		5’GACTCCGATGGTGCTGTGG3’