Figure 6. KLF11 inactivation does not affect liver function but increases hepatocyte apoptosis.

(A) In a subset of olive oil and CCl₄-treated KLF11^-/- and wild type mice, sera were screened for a panel of liver injury markers (as detailed in results). No differences were found between wild type and knockout animals under CCl₄ treatment for the displayed markers. A significant difference was found between wild type and knockout animals under olive oil treatment owing to a previously characterized defect in fatty acid oxidation in the absence of KLF11. * = p<0.05. (B) Immunohistochemistry for cleaved caspase 3 and TUNEL quantification reveals that CCl₄-treated KLF11^-/- experienced increased apoptosis, exclusive to hepatocytes, compared to CCl₄-treated wild type animals. * = p<0.05. ** = p<0.01. All images appear at X200 magnification with scale bar equaling 200µm.