Table 2. Oligonucleotide primers used in RT-PCR and sequencing.
| Primer | Nucleotide position(nt)a | Primer sequence(5’–3’) | Orientation | Reference |
|---|---|---|---|---|
| VP3F1 | 1419-1439 | GCTAGGTTTACAGATTTGGAG | Forward | This study |
| VP3R1 | 2408-2388 | TGTCTCAGGCACTTTCTTTGC | Reverse | This study |
| VP3NF2 | 1452-1472 | ACTCCTCTTTCTACACAGATG | Forward | This study |
| VP3NR2 | 2399-2379 | CACTTTCTTTGCTAAAACTGG | Reverse | This study |
| VP1F1 | 2167-2192 | GTTTTGCTCCTCTTTATCATGCTATG | Forward | [12] |
| VP1R1 | 3384-3363 | CATCCATCTCAAGAGTCCACAC | Reverse | This study |
| VP1NF2 | 2181-2200 | TATCATGCTATGGATGTTAC | Forward | This study |
| VP1NR2 | 3286-3267 | TTCATTATTTCATGCTCCTC | Reverse | [12] |
a Position related to the genome of HAV strain HM175 (M14707)