Table 4. Characterization of the mutant spectrum of HAV isolates from clinical samples in this study.
| Genomic region/Isolates | Mutationsa/Nucleotides sequenced | Nucleotide mutation frequencyb | Amino acid mutation frequencyb | ||
|---|---|---|---|---|---|
| SN C | SNA d | ||||
| VP3/HtXj15.06 | 26/14760 | 1.76x10-3 | 3.25x10-3 | 0.82 | 0.72 |
| VP3/PyNx21.07 | 14/14760 | 9.49x10-4 | 1.63x10-3 | 0.67 | 0.50 |
| VP3/SjzHb19.05 | 29/14760 | 1.96x10-3 | 2.44x10-3 | 0.91 | 0.56 |
| VP1/HtXj15.06 | 37/18000 | 2.06x10-3 | 3.17x10-3 | 0.95 | 0.77 |
| VP1/PyNx21.07 | 13/18000 | 7.22x10-4 | 1.83x10-3 | 0.56 | 0.50 |
| VP1/SjzHb19.05 | 36/18000 | 2.00x10-3 | 2.67x10-3 | 0.98 | 0.77 |
| VP1-2A/HtXj15.06 | 18/7740 | 2.33x10-3 | 5.04x10-3 | 0.75 | 0.59 |
| VP1-2A/PyNx21.07 | 6/7740 | 7.75x10-4 | 1.55x10-3 | 0.38 | 0.26 |
| VP1-2A/SjzHb19.05 | 16/7740 | 2.07x10-3 | 3.88x10-3 | 0.59 | 0.45 |
a Mutant residues are those that vary relative to the corresponding consensus sequences
b The nucleotide mutation frequency is the total number of mutations divided by the total number of nucleotide sequenced. The amino acids mutation frequency is the total number of nonsynonymous mutations divided by the number of amino acids encoded in the sequence analyzes.
C The normalized Shannon entropy is calculated as SN= - [∑i (pi lnpi)] /lnN, in which pi is the proportion of each sequence of the mutant spectrum and N is the total number of sequences compared.
d The normalized Shannon amino acid entropy was calculated as SNA= - [∑i (qi lnqi)] /lnN, where qi is the frequency of each amino acids sequence of the mutant spectrum and N is the total number of sequences compared.