Table 1. Host proteins that were detected in the HCV 3’ X complex identified by 2D/MASS.
| SwissProtaccession# | ProteinName | Mass(kDa) | pIa | Percentpeptidecoverage | Zbscore | Knownfunctionand/orpossibleroleinHCVreplicationc |
|---|---|---|---|---|---|---|
| P08758 | Annexin 5 | 35.96 | 4.9 | 27 | 1.33 | Anti-apoptosis, signal transduction (?) |
| A8K1K1 | Carnosine dipeptidase 1 | 56.79 | 5.1 | 15 | 2.43 | Proteolysis (?) |
| P11498 | Pyruvate carboxylase | 130.37 | 6.4 | 51 | 2.4 | Gluconeogenesis, lipid biosynthesis (?) |
| Q969R2 | KIAA1664 (ORP4) | 100.75 | 6.1 | 9 | 2.43 | Lipid metabolism, vesicle transport, signaling (?) |
| Q9UHL0 | DEAD/H box polypeptide 25 | 55.06 | 5.9 | 7 | 1.82 | RNA helicase (?) |
| Q8WVW5 | Actin gamma | 40.83 | 5.8 | 40 | 2.43 | Cell motility (?) |
| Q01581 | HMG-CoA synthase | 57.85 | 5.2 | 21 | 2.43 | Cholesterol and isoprenoid syntheses, lipid metabolism (25) |
| P22735 | Transglutaminase 1 | 90.12 | 5.7 | 10 | 2.43 | Protein modification, cell envelope biogenesis (?) |
| Q14532 | Keratin | 51.78 | 4.8 | 17 | 2.43 | Epidermis development (?) |
| Q9NZD9 | Tumor necrosis factor receptor | 14.72 | 5.7 | 45 | 0.75 | Apoptosis, signal transduction (?) |
| Q9HC85 | Metastasis-related protein | 10.40 | 5.4 | 59 | 1.27 | Unknown (?) |
a) pI is the isoelectric point of each protein.
b) The Z score calculated by ProFound® search engine and is an indicator of the search result quality. It is estimated when the search result is compared against an estimated random match population and is the distance to the population mean in units of standard deviation.
c) Known functions of each protein are derived from the literature and used to indicate their possible roles in HIV replication, which are marked as in the table.