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. 2013 Sep 17;8(9):e75742. doi: 10.1371/journal.pone.0075742

Figure 3. Conventional PCR targeting a 133 bp fragment of Y. pestis pla gene.

Figure 3

A) Amplification products of the synthetic pla construct at concentrations of 10, 5 and 1 copy, respectively, are shown. Three samples were extracted from ancient bones originating from a modern time cemetery lacking any Y. pestis specific amplicons (bone matrix).

B) Besides the synthetic pla construct, the gel shows products of PCR reactions set up, containing 1 ng DNA of each from the negative panel organisms (Table 2).

C) Gel showing amplification products of aDNA extracted from teeth (Table 1). M: marker, PC: positive control, NTC: no template control, MMC: master mix control, WC: water control, MP: Manching-Pichl, Germany, origin of individuals, E1-8: extraction controls, Ba: Basel, Switzerland, origin of individuals, B: Brandenburg, Germany, origin of individuals.