Table 1. The investigated ancient samples originated from three different burial sites.
| Burial site | Age (A.D.) | Individuals tested (positive) in this study | Positive individual | Quantitative screening PCR targeting pla (70 bp) | Maximum pla gene copies per 1µl | Specific pla amplicons & sequence (133 nt) | Specific caf1 amplicons & sequence (161nt) |
|---|---|---|---|---|---|---|---|
| Manching-Pichl, Germany | 1250-1500 | 20 (4) | |||||
| MP17-I | 4/4 | 560 | 3/3 | 2/3 | |||
| MP19-II | 4/4 | 700 | 3/3 | 1/2 | |||
| MP59-I | 4/4 | 22 | 3/3 | 1/3 | |||
| MPS01-I | 4/4 | 3 | 1/3 | 0/2 | |||
| Brandenburg, Germany | 1640 | 3 (3) | |||||
| B1 | 1/4 | ≤ 1 | 0/3 | n.d. | |||
| B2 | 2/4 | 2 | 0/3 | n.d. | |||
| B3 | 4/4 | 6 | 2/3 | 1/2 | |||
| Basel, Switzerland | 1300-1490 | 6 (0) | neg | ||||
| 13 extraction controls | 0/4 | 0/3 | n.d. |