Table 5. Probes and primers used for real-time PCR-based method.

Name	Fluorophore	Sequence	Quencher
1a66	6-FAM	5’-CAGCTTGAACAGGC-3’ MGB	NFQ-MGB
1b266	VIC	5’-CAACTTGAAAAAGC-3’ MGB	NFQ-MGB
F56_1	-	5’-CACACTCCAGTYAAYTCCTGG -3’	-
R56_1	-	5’-CWMCTGGAGAGTAACTGTGGAG -3’	-

Probes and primers used for HCV subgenotypes 1a and 1b in HCV subgenotyping assay by real-time PCR-based method in the HCV NS5B region [12]. Probes 1a66 and 1b266 were used for the detection of HCV subgenotypes 1a and 1b, respectively.