Table 5. Probes and primers used for real-time PCR-based method.
Name | Fluorophore | Sequence | Quencher |
---|---|---|---|
1a66 | 6-FAM | 5’-CAGCTTGAACAGGC-3’ MGB | NFQ-MGB |
1b266 | VIC | 5’-CAACTTGAAAAAGC-3’ MGB | NFQ-MGB |
F56_1 | - | 5’-CACACTCCAGTYAAYTCCTGG -3’ | - |
R56_1 | - | 5’-CWMCTGGAGAGTAACTGTGGAG -3’ | - |
Probes and primers used for HCV subgenotypes 1a and 1b in HCV subgenotyping assay by real-time PCR-based method in the HCV NS5B region [12]. Probes 1a66 and 1b266 were used for the detection of HCV subgenotypes 1a and 1b, respectively.