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. Author manuscript; available in PMC: 2014 Sep 15.
Published in final edited form as: Cancer. 2013 Jun 21;119(18):3411–3418. doi: 10.1002/cncr.28203

Figure 3. Pathologic examination of the tumor border.

Figure 3

A. After resection and slicing of the same specimen, the rim around the tumor can be visualized ex vivo.

B. Shown are hematoxylin and eosin (HE) staining with a pseudo-colored green NIR fluorescence overlay of a 20 µm tissue section of a colorectal liver metastasis using a 5 × objective. The fluorescent rim in stromal tissue appears in the transition zone between tumor (T) and normal liver tissue (L).

C. Shown is liver tissue located in the fluorescent rim of a colorectal liver metastasis. Consecutive frozen sections (5 µm) are stained with hematoxylin and eosin (HE) and for CD68, CD31, and CK7. Microscopic color images (left column), NIR fluorescence images (middle column), and a pseudo-colored green merge (right column) were obtained (100× zoom). The NIR fluorescence signal is mainly located intracellularly and shows a high correlation with CK7 staining.