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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Kidney Int. 2013 Jun 5;84(5):1041–1046. doi: 10.1038/ki.2013.202

Figure 4. Generation of primary proximal tubular cell culture using the Terminator mouse.

Figure 4

A. Primary cultures of proximal tubular cells derived from Ggt-Cre;Rosa-DTRflox mice were treated ± DT for 96 hours, lysed and subjected to Western analysis of megalin and Wt1 expression; B, fluorescent immunostaining of Ecadherin (epithelial marker), Desmin (interstitial/mesangial marker), Wt1 (podocyte marker) and THP protein (thick ascending limb marker) expression by passage zero cells treated as in (A).