Table 1.
Comparisons of passive and evoked membrane electrical properties of bipolar- and pyramidal-shaped induced neurons between control and DS patients.
| Group | Resting Vm (mV) |
Input Resistance (GΩ) |
VThreshold (mV) |
AP Amplitude (mV) |
AP Frequency (Hz) |
|---|---|---|---|---|---|
| B-Ctrl | −67.15 ± 1.37 (33) |
1.25 ± 0.15 (33) |
−23.67 ± 1.51 (30) |
114.90 ± 4.69 (34) |
3.48 ± 0.73 (27) |
| B-DS | −58.55 ± 1.28** (45) |
1.24 ± 0.12 (45) |
−30.83 ± 1.53** (37) |
121.18 ± 3.26 (37) |
11.6 ± 1.33** (37) |
| P-Ctrl | −63.05 ± 2.72 (9) |
1.02 ± 0.34 (9) |
−20.51 ± 3.37 (8) |
125.38 ± 5.14 (8) |
5.10 ± 2.66 (7) |
| P-DS | −58.08 ± 1.24 (31) |
1.02 ± 0.17 (31) |
−37.32 ± 1.51** (26) |
114.35 ± 3.97 (26) |
12.64 ± 1.99* (24) |
Values are mean ± SEM; cell numbers in parentheses.
Abbreviations: Resting Vm, resting membrane potential; VThreshold, threshold membrane potential for evoking an action potential (AP); AP Amplitude, Spike height measured from the resting membrane potential level to the peak; AP Frequency, the frequency of repetitive firing of a given iPSC in response to injection of a 1500 ms depolarizing current pulse (0.02 nA); B-Ctrl, bipolar-shaped neurons from control iPSCs; B-DS, bipolar-shaped neurons from DS iPSCs; P-Ctrl, pyramidal-shaped neurons from conrol iPSCs; P-DS, pyramidal-shaped neurons from DS iPSCs.
p<0.05;
P < 0.001,
Student’s t test or Mann-Whitney Rank Sum test.