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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Virol Methods. 2013 Jun 19;193(2):270–277. doi: 10.1016/j.jviromet.2013.06.008

Figure 3. Efficacy and Viability of AAV2 transfection and infection in vitro in 293T cells.

Figure 3

(A) 72-hours post-transfection using protocol above with pHelper, pAAV-GFP and pAAV-R2C2, showing high efficiency. (B) Same as Figure A in brightfield, without GFP filter. Approximately 20 percent cell death observed. (C) 293T cells infected with purified AAV2-GFP virus, MOI=3.