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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Neurobiol Dis. 2013 Jul 2;59:1–17. doi: 10.1016/j.nbd.2013.06.013

Figure 10. ELS does not mediate alterations in localization of S6K, but does mediate changes in S6K–P at P60.

Figure 10

A, S6K was not significantly removed from the cytosolic compartment, though a trend similar to PP2A and FRMP was suggested (S2 fraction, ELS: 82.01 ± 9.45%, n = 14; control: 100 ± 10.50%, n = 10, P = NS, Student’s t-test). S6K P2 (synaptosomal) fraction was not changed (ELS: 121.90 ± 12.18%, n = 14, control: 100 ± 18.58%, n = 10, P = NS, Student’s t-tests). B, Phosphorylated S6K (Thr 389) was significantly reduced in the cytosolic compartment (S2 fraction, ELS: 63.13 ± 10.35%, n = 10; control: 100 ± 13.16%, n = 8, P < 0.02, Student’s t-test). Phosphorylated S6K (Thr 389) was significantly increased in the P2 (synaptosomal) fraction (ELS: 175.00 ± 38.58%, n = 11, control: 100 ± 13.04%, n = 10, P < 0.05, Student’s t-tests). Semi-quantitative western-blot technique(Cornejo et al., 2007) was used to determine immunoreactivity/mg loaded protein, which were normalized to controls (see Methods). Typical blots with equivalent protein loading shown. Solid bars ELS, open bars control.