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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Stem Cells. 2013 Aug;31(8):1669–1682. doi: 10.1002/stem.1416

Figure 2. Abi3bp regulates MSC differentiation and motility.

Figure 2

(A) Osteogenesis. The three wild-type and Abi3bp knockout MSC isolations were evaluated for alizarin red deposition following osteogenic differentiation, n=5, scale bar 200 microns. Alizarin red staining was normalized to cell number using an MTS assay n=5. *** p≤0.001. Right: RNA, extracted at this time point, was analyzed for Runx2 expression (n=3) *** comparison between vehicle and induced p≤0.001, ‡comparison between WT and KO induced cells p≤0.05. (B) Chondrogenesis. Wild-type and Abi3bp knockout MSC isolates were stained with alcian blue following differentiation, n=3, scale bar 100 microns. Alcian blue staining was normalized to cell number using an MTS assay, n=5. *** p≤0.001 comparison between vehicle and induced (C) Wild-type and Abi3bp knockout MSCs were differentiated to smooth muscle, n=5, scale bar 100 microns. (D) Adipogenesis. MSCs from the wild-type and Abi3bp knockout isolations were stained with Oil Red following differentiation, n=5, scale bar 100 microns. Oil Red staining was normalized to cell number using crystal violet, n=5. *p≤0.001 comparison between vehicle and induced, *** p≤0.001, * p≤0.05. ‡comparison between WT and KO induced cells, ‡‡‡p≤0.001, ‡‡p≤0.01. (E) MSC-GFP-Akt1 cells expressing either scrambled or Abi3bp shRNA were used in a scratch assay. The width of the scratch was measured at t=0h and t=14h (N=9).