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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Virol Methods. 2013 Jun 26;193(2):332–336. doi: 10.1016/j.jviromet.2013.06.020

Figure 1. Non-specific interaction between HRP-Conjugating 2nd antibody and monocolonal anti-p30 antibody.

Figure 1

(A) HRP conjugated anti-goat 2nd antibodies were tested with varying concentrations of CRL-1912 monoclonal antibody coating the ELISA trays. Filled circles, rabbit anti-goat antibody; open circle, donkey anti-goat antibody; filled triangles, bovine anti-goat antibody. (B–D). Dilution of bovine anti-goat antibody in the presence of CRL-1912 monoclonal antibody and MuLV p30 recombinant protein. Panel B, 1:5000 dilution; Panel C, 1:10,000 dilution; Panel D, 1:20,000 dilution of bovine anti-goat antibody. Closed circles, absence of CA; open circles, 20 ng/nl CA.