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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: J Virol Methods. 2013 Jun 26;193(2):332–336. doi: 10.1016/j.jviromet.2013.06.020

Table 2.

Quantitation of Pr65Gag versus P30 CA

MuLV CA conc. (μg/ml)1 vRNA copy number2
Wildtype 1.75±0.171 1.88±0.16 ×109
WT+Amprenavir 1.31±0.197 2.77±0.07 ×109
1

CA concentration determined by ELISA.

2

Vial RNA was purified by QIAamp Viral RNA mini kit (Qiagen, Valencia CA USA) and cDNA synthesis using Superscript III Reverse Transcriptase primed with Random Hexamers (Invitrogen, Carlsbad, CA USA). vRNA was quantified as previously described using the primers set for minus strand strong stop (MSSS) intermediates (Schneider et al., 2012).