. Author manuscript; available in PMC: 2014 Jan 1.

Published in final edited form as: Methods Appl Fluoresc. 2013;1(2):025001. doi: 10.1088/2050-6120/1/2/025001

Table 1.

Summary of results from a variable temperature study of the time-resolved and steady-state fluorescence anisotropy of anti-rabbit IgG and an anti-rabbit IgG - rabbit IgG complex.

	antiIgG	antiIgG-IgG	Δ^c
r₀	0.36 ± 0.001
r₀^app	0.18 ± 0.002	0.19 ± 0.004	0.01
r_{500 nm} (20°C)	0.11 ± 0.004	0.13 ± 0.005	0.02 (+18 %)
θ₁ (20°C)	52 ± 15 ns	91 ± 30 ns	39 ns (+75 %)
M^a	~150,000 Da	~300,000 Da
M_Perrin^b	187,900 ± 18% Da	280,900 ± 18% Da	107,000 Da
M_θ^b	150,500 ± 1% Da	258,700 ± 1% Da	108,200 Da

Masses taken from ref ²⁶.

Masses calculated assuming antiIgG and antiIgG-IgG are spherical rotors with zero net hydration, see text for detail.

difference between the free antiIgG and the complex.