Figure 2.

MEK1/2 inhibition suppresses TMX toxicity on O-2A/OPCs. A, Protective agents were identified in cultures of O-2A/OPCs coexposed to 1 μm TMX and an individual drug from the NINDS-II drug library for 48 h. Cell death was measured by percentage of PI+ cells in the total cell pool. B, C, O-2A/OPCs treated with DMSO or 1 μm TMX were coexposed to increasing concentrations of U0126 (B) or AZD6244 (C) for 48 h. Viable cell number was determined by Calcein-AM+ cell counting with a Celigo adherent cell cytometer. Error bars indicate SEM.