Figure 3.

Differential effects of MEK1/2 inhibition on O-2A/OPCs and breast cancer cells. A, MCF7 cells were coexposed to TMX and 2.5 μm U0126, 2.5 μm AZD6244, or 1 μm β-estradiol for 4 d. AlamarBlue was used to measure viability of MCF7 cells as their ability to grow as aggregates precluded accurate Celigo-based analysis with Calcein-AM and PI. B, C, O-2A/OPCs were dosed with 1 μm TMX ± 2.5 μm U0126, 2.5 μm AZD6244, or 1 μm β-estradiol for 48 h, after which live cell number (B) and cell death (C) were determined by Calcein-AM and PI analyses, respectively. One-way ANOVA followed by Bonferroni pairwise comparison was performed. Data represent the mean ± SEM, normalized to control, N ≥ 3. *p < 0.05; **p < 0.01; ***p < 0.001. NS, Not significant.