Figure 7.

COX-1-specific inhibition does not attenuate inflammatory responses to LPS challenge. Expression of IL-1β, TNF-α, IL-6, and CXCL-1 was measured in the plasma and the hippocampus and thalamus of ME7 animals 2 h after challenge with saline or LPS (100 μg/kg), in the presence or absence of SC-560 (30 mg/kg). Plasma levels of proinflammatory cytokines were measured using DuoSet ELISA kits and brain transcription of proinflammatory cytokine transcripts using quantitative PCR. There was a significant main effect of challenge by two-way ANOVA, with post hoc Bonferroni tests indicating a significant effect of LPS: *p < 0.05, **p < 0.01, ***p < 0.001 with respect to saline-challenged control, n = 5 per group with the exception of ME7+saline+SC-560 (n = 4). All data have been presented as the mean ± SEM.