Figure 7.

Average difference scores of the number of spikes observed during the trace interval before and after DCN inactivation for mPFC persistent (A), trace (B), and phasic tone (C) cell response types, and difference scores for the expression of CRs within the sessions in which those cells were recorded. A, Difference scores derived from the average number of spikes observed during the stimulus-free trace interval between pre-DCN and post-DCN infusion epochs (red bars) were not significantly different from controls (gray bars) for persistent cells (left: n = 8 and 14 cells, t = 0.34, p = 0.74), even though a significant difference in CRs was observed for sessions in which those cells were recorded (right: n = 6 and 12 sessions, t = 15.77, p < 0.001). B, In contrast, trace interval cells showed a significant decrease in the post-pre difference in the average number of spikes observed between DCN infusion and control sessions (left: n = 16 and 16 cells, t = 2.51, p = 0.02), as was also observed for the change in behavioral responses from those sessions (right: n = 10 and 9 sessions, t = 13.58, p < 0.001). C, As a within manipulation control, phasic tone cells were analyzed for differences in post-pre average spike responses during the tone CS and showed no significant difference between control and DCN inactivation sessions (left: n = 15 and 25 cells, t = 0.62, p = 0.54) even though the behavioral responses were different during the corresponding sessions (right: n = 9 and 14 sessions, t = 14.07, p < 0.001). *Bonferroni-corrected, p < 0.017.