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AT1 receptor gene amplification in 2 % agarose gel stained with Sybr®Safe (Invitrogen). 1, 3, and 5 Positive amplification of cDNA from control (sham operated) rats, and rats subjected to CHP and chronic cerebral hypoperfusion + candesartan (CHP+cand), respectively. 2, 4, and 6 The corresponding negative controls without reverse transcription, which show negative amplification
