Fig. 6.

Real-time quantitative RT-PCR (a), western blot (b), and Lucigenin enhanced chemiluminescence (c) analysis of the NADPH complex activation in the ventral mesencephalon of control rats as compared with rats subjected to CHP or chronic cerebral hypoperfusion and treatment with the AT1 blocker candesartan (CHP + cand). Protein expression was obtained relative to the GAPDH band value and the expression of each gene was obtained relative to the housekeeping transcripts (β-actin). The results were then normalized to values for control rats (100 %). NADPH oxidase activity (c) was expressed as relative light units (RLU/min/mg protein). Data are means ± SEM. *p < 0.05 relative to the control group, #p < 0.05 relative to the group subjected to chronic hypoperfusion (one-way ANOVA and Bonferroni post hoc test)