Fig. 2.

Age- and CR-related changes of the relative mtDNA content and of the 4.8-kb deletion content. a The histogram shows the mean value of the ratio mtDNA/nuclear DNA in AL and CR rats, compared to young rats. Bars represent the mean (±SD) obtained, respectively, from analysis in triplicate of total nucleic acids from each young and aged AL and CR-treated rat. *p < 0.05 versus the value of the young rats (fixed as 1); n number of analyzed animals. b The histogram shows the mean value of the ratio deleted mtDNA/total mtDNA in AL and CR rats, compared to young animals. Bars represent the mean (±SD) of the values obtained, respectively, from analysis in triplicate of the total nucleic acids from each young and aged AL and CR-treated rat. *p < 0.05 versus the value of the young rats (fixed as 1); n number of analyzed animals