Fig. 2.

Analysis of SIRT1, COL2A1, and aggrecan expression in human NP cells by real-time PCR and Western blotting. a The values of real-time PCR for SIRT1, COL2A1, and aggrecan gene expression obtained from patients with LVF were normalized versus those from patients with DDD. b Western blotting analysis of SIRT1, COL2A1, and aggrecan protein expression in human NP cells from LVF and DDD. c–e Quantitative analysis of the Western blotting was performed with the software. The data are mean of three different experiments; SD. Asterisk: significant difference with respect to LVF (p < 0.05); number sign: significant difference with respect to DDD (p < 0.05)