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. 2012 Sep 19;35(5):1741–1753. doi: 10.1007/s11357-012-9474-y

Fig. 6.

Fig. 6

Phosphorylated Akt (p-Akt), Akt, active caspase-3, and Bcl-2 in degenerative human NP cells detected by Western blotting. a The amounts of phosphorylation of Akt at Ser473 increased when treated with resveratrol. a Treatment with resveratrol + LY294002 (10 μmol/L) or SIRT1 siRNA inhibited phosphorylation of Akt at Ser473. Conversely, resveratrol attenuated active caspase-3, and resveratrol + LY294002 (10 μmol/L) or SIRT1 siRNA active caspase-3. a Different treatments did not cause obvious changes in the amount of Bcl-2 in total cell lysates. b, c The relative density of p-Akt and active caspase-3 is expressed as the ratio (p-Akt/GAPDH, active caspase-3/GAPDH). d–f Apoptotic rate was measured by flow cytometry after treatment with resveratrol + LY294002 or Akt-siRNA (see also Fig. 5b). Data represents mean ± SD of three independent experiments. Asterisk: significant difference with respect to control or resveratrol + LY294002 group (p < 0.05). Number sign: significant difference with respect to NC-siRNA group (p < 0.05). Asterisk and number sign: significant difference with respect to control, resveratrol + LY294002, or Akt siRNA group (p < 0.05)