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. 2012 Sep 14;35(5):1755–1765. doi: 10.1007/s11357-012-9475-x

Fig. 4.

Fig. 4

Effect of chronic caffeine intake on Glut4, AMPKα1, and AMPKα1 Thr172 phosphorylation expression in skeletal muscle in 3-, 12-, and 24-month-old rats. a–c Western blot comparing Glut4, AMPKα1, and AMPKα1 Thr172 phosphorylation immunoreactivity in skeletal muscle, corresponding to 54, 63, and 63 kDa bands, respectively, when comparing 3-, 12-, and 24-month-old rats with and without caffeine intake; d–f the average relative Glut4, AMPKα1, and AMPKα1 Thr172 phosphorylation immunoreactivity in the different paradigms expressed in relation to GAPDH immunoreactivity. *p < 0.05, **p < 0.01, ***p < 0.001, compared with 3-month-old rats without caffeine intake; #p < 0.05, compared with values without caffeine within groups (two-way ANOVA with Bonferroni multicomparison test, comparing all the groups with the control group). Data are presented as the means ± SEM; Glut4 expression (without caffeine n = 6–10; with caffeine n = 7–10); AMPK and AMPKα1 Thr172 phosphorylation expression (without caffeine n = 4–18; with caffeine n = 4–8)