. 2013 Aug 29;9(4):745–751. doi: 10.5114/aoms.2013.37274

Table II.

Primer used for DNA amplification of EBV genome

No.	Target genes		Sequences (5’ to 3’)	Region of amplification	Annealing temp.[°C]	Expected product [bp]
1	GAPDH	GAPDH 3S	GGCCTCCAAGGAGTAAGACC CCCCTCTTCAAGGGGTCTAC		55	157
1	GAPDH	GAPDH 3AS	GGCCTCCAAGGAGTAAGACC CCCCTCTTCAAGGGGTCTAC		55	157
2	EBER 2	EBER-2S	CCCTAGTGGTTTCGGACACA ACTTGCAAATGCTCTAGGCG	6969–6988 7075–7056	60	108
2	EBER 2	EBER-2AS1	CCCTAGTGGTTTCGGACACA ACTTGCAAATGCTCTAGGCG	6969–6988 7075–7056	60	108
3	EBNA 2	E2 up	AGGCTGCCCACCCTGAGGAT GCCACCTGGCAGCCCTAAAG	48170–48189 48339–48320	58	170 or 189
3	EBNA 2	E2 low	AGGCTGCCCACCCTGAGGAT GCCACCTGGCAGCCCTAAAG	48170–48189 48339–48320	58	170 or 189
4	BNLF-1	LMP2CS	CTAGCGACTCTGCTGGAAAT GAGTGTGTGCCAGTTAAGGT	168373–168392 168075–168056	55	307 or 337
4	BNLF-1	LMP2CAS	CTAGCGACTCTGCTGGAAAT GAGTGTGTGCCAGTTAAGGT	168373–168392 168075–168056	55	307 or 337
5	Gp220	Primer 1	GGCTGGTGTCACCTGTGTTA CCTTAGGAGGAACAAGTCCC	BamHIL region	55	239
5	Gp220	Primer 2	GGCTGGTGTCACCTGTGTTA CCTTAGGAGGAACAAGTCCC	BamHIL region	55	239