Fig. 3.

SC1 and PRMT5 are found in the nucleus and the cytoplasm of early differentiating neurons. NSCs isolated at E10.5 were cultured in the presence of NT3 to promote neurogenesis. After 3 days in vitro, the cells were immunolabelled with antibodies to visualize PRMT5, SC1 and TuJ1 to identify early-born neurons. Merged images showing Hoechst staining of DNA are shown on the right. Magnified images of the boxed areas from merged panels highlight (white arrowheads) the presence of SC1 and PRMT5 in both perinuclear and axonal parts of young neurons. Scale bar is 25 μm.