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Randomization of the internal DARPin repeat. The second internal repeat (IR2) of the original (off7, left panel, PDB 1SVX) and new (C7_16, right panel, PDB 4JB8) library is shown in the context of the N3C DARPin (A) and in isolation (B). Diversified positions are highlighted as colored side chains shown as sticks. The repeat sequences and the permitted residues of the randomized positions are given in (C).
