Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 16;202(6):875–886. doi: 10.1083/jcb.201305094

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Xu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — Translocation of PS to the cytosolic leaflet by a flippase is required for protein transport between the EE and the TGN. (A) Dnf1 N550S and wild-type (WT) Drs2 restored normal Snc1 trafficking in drs2Δ cells, whereas Drs2 [QQ → GA] and WT Dnf1 failed. Arrowheads indicate Snc1 plasma membrane localization polarized to buds. Bar, 5 µm. (B) Three independent colonies of each strain were cultured to mid-log phase and Snc1 localization was quantified. (C) Dnf1 N550S but not Drs2 [QQ → GA] restored resistance of chs6Δdrs2Δ cells to CW. (D) Quantitation of CW fluorescent staining of strains used in C. Error bars indicate mean + SD. (E) The strains indicated were pulse-labeled with [³⁵S]methionine for 10 min and chased for 3 h at 20°C. CPY or ALP were immunoprecipitated from cells collected at 0, 1, and 3 h of chase and subjected to SDS-PAGE. WT Drs2, Drs2 [QQ → GA], and Dnf1 N550S restored normal CPY and ALP transport kinetics, whereas WT Dnf1 partially suppressed the transport defects. pro, precursor; m, mature.