Figure 5.

The RacGAP1–IQGAP1 complex promotes integrin-dependent invasive migration. (A) A2780 cells were subjected to control or IQGAP1 #1 RNAi and seeded onto CDMs. Images were captured, and pseudopod length was determined as in Fig. 2 (A and B; n > 100/condition). (B) A2780 cells were treated as in A and seeded into inverted invasion assays in the presence or absence of FN and cRGDfV as indicated. (C) A2780 cells stably expressing GFP, RacGAP1^WT, RacGAP1^249A, or RacGAP1^249D were seeded onto CDMs and stimulated with cRGDfV as indicated, images were captured, and pseudopod length was measured as in Fig. 2 (A and B; n > 40/condition). (D) A2780 cells as in C were seeded into inverted invasion assays in the presence of FN and stimulated with cRGDfV as indicated. (E) A2780 cells stably expressing GFP, RacGAP1^WT, or RacGAP1^249D were transfected as in A and seeded into inverted invasion assays in the presence of FN and cRGDfV. Yellow lines indicate the level of invasion or pseudopod length under control conditions. Bars, 50 µm. Data represent means ± SEM from at least three independent experiments. *, P < 0.05; ***, P < 0.001.