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. 2013 Sep 16;202(6):849–859. doi: 10.1083/jcb.201302131

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© 2013 Choi et al.

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Figure 3. — His58 confers pH-dependent FAK-pY397. (A) Structure of avian FAK (PDB 2J0J) showing His41 and His75 in the F1 lobe of the FERM domain at the interface with the kinase domain and His58 in close proximity to the interdomain linker. (B) Left immunoblot shows attenuated pY397 in wild-type FAK at pH 6.5 is restored in FAK-H58A. Right histogram shows abundance of pY397 relative to wild-type FAK at pH 7.5 (means ± SEM; n = 3). (C) Time-dependent increase in pY397 of FERM H58A in trans by the kinase domain is pH independent. (D) Time-dependent increase in pY397 of FERM H41A and H75A in trans by the kinase domain is pH dependent.