Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 16;202(6):951–966. doi: 10.1083/jcb.201301081

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — MR and uPARAP facilitate intracellular collagen degradation in vivo. (A) Western blot of MR (lanes 1 and 2) and uPARAP (lanes 3 and 4) in protein extracts from dermis of MR-deficient (Mrc1^−/−; lane 1) and a wild-type (WT) littermate (lane 2) or uPARAP-deficient (Mrc2^−/−; lane 3) and a wild-type littermate (lane 4). Arrows on the right show the positions of MR and uPARAP. Positions of molecular mass markers (kilodaltons) are indicated on the left. Specificity of MR and uPARAP detection is shown by the absence of immunoreactivity in Mrc1^−/− and Mrc2^−/− mice, respectively. (B–D) Representative examples of collagen uptake in cells from the dermis of wild-type (B), Mrc1^−/− (C), and Mrc2^−/− (D) mice 24 h after collagen injection. Bars, 10 µm. Arrows in B and D show examples of collagen in lysosomal vesicles. (E) Percentage of cells in collagen- and dextran-injected wild-type (n = 5), Mrc1^−/− (n = 3), and Mrc2^−/− (n = 5) dermis that internalize collagen or dextran. *, P < 0.05; and **, P < 0.01 relative to collagen-internalizing cells in wild-type mice (Student’s t test, two tailed). (F) Enumeration of cells present in noninjected or collagen-injected dermis of wild-type (n = 3), Mrc1^−/− (n = 3), and Mrc2^−/− (n = 3) mice. Error bars show means ± SD.