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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Epigenomics. 2013 Apr;5(2):131–146. doi: 10.2217/epi.13.1

Figure 3. Sectional in situ hybridization with Shh and Sox4 riboprobes on sagittal sections of GD14 oral/facial region and palatal processes.

Figure 3

(A) Sagittal sections of oral/facial region from GD14 mice probed with Sox4 sense and antisense riboprobes. Representative photomicrographs indicate that Sox4 expression, in the secondary palate, is specifically localized to the rugae of the palate on GD14. (B) Sagittal sections of secondary palates from GD14 mice probed with Sox4 and Shh antisense riboprobes. Similar to Shh (which is expressed in the rugae epithelium to direct anteroposterior extension of the palate), Sox4 is also found to be expressed in the epithelial thickenings of the palatal rugae.