Table 1.
Characterisation of the CPAP:STIL interaction in vitro
| Danio rerio STIL peptide in syringe | Danio rerio CPAP937–1124 TCP domain in cell | Number of binding sites (N) | SD N | KD (μM) | SD KD (μM) | ΔH (kcal/mol) | SD (kcal/mol) | n (number of measurements) | Factor change in KD |
|---|---|---|---|---|---|---|---|---|---|
| STIL404–448 | WT | 1.07 | 0.04 | 1.9 | 0.2 | −10.1 | 0.3 | 5 | 1 |
| STIL411–428 | WT | 0.98 | 0.03 | 4 | 0.3 | −11.3 | 0.5 | 3 | 2 |
| STIL429–448 | WT | 0.97 | 0.08 | 540 | 130 | −6.3 | 0.6 | 2 | ∼280 |
Binding parameters between D. rerio CPAP and various D. rerio STIL constructs obtained from ITC experiments. Fitting was performed with N as a variable. Constraining N to a fixed value of 1 during fitting produced KD values that were within the experimental error of those tabulated here.