Table 2.
Native dataset analysis and refinement statistics
| D. rerio CPAP937–1124 WT | D. rerio CPAP937–1124 E1021V | D. rerio CPAP937–1124 + D. rerio STIL408–428 complex | D. melanogaster dSTIL1–47 − dCPAP700–901 fusion complex | |
|---|---|---|---|---|
| Beamline | Diamond I02 | MRC-LMB Cambridge UK | Diamond I04 | Diamond I04 |
| Space group | P21 | P21 | P21 | P1 |
| Wavelength (Å) | 0.9786 | 1.5418 | 0.9795 | 0.9795 |
| Monomers in the asymmetric unit | 1 | 1 | 2 | 3 |
| Unit cell dimensions (Å) | a = 52.34; b = 36.44; c = 56.44; α = 90.00; β = 117.31; γ = 90.00 | a = 52.12; b = 36.48; c = 56.46; α = 90.00; β = 117.47; γ = 90.00 | a = 60.25; b = 67.47; c = 61.65; α = 90.00; β = 113.92; γ = 90 | a = 58.64; b = 69.91; c = 69.98; α = 86.96; β = 88.64; γ = 67.69 |
| Resolution (Å) | 29.48–1.7 | 36.48–1.9 | 56.35–2.7/2.2 (anisotropy) | 64.60–2.57 |
| Completeness(overall/inner/outer shell) | 99.7/99.4/100 | 100/99.6/100 | 99.9/99.5/99.9 | 97.6/93.6/97.2 |
| Rmerge (overall/inner/outer shell) | 0.074/0.030/0.929 | 0.096/0.028/1.093 | 0.101/0.053/1.008 | 0.091/0.069/0.512 |
| Rpim (overall/inner/outer shell) | 0.029/0.012/0.369 | 0.039/0.012/0.456 | 0.050/0.027/0.505 | 0.061/0.035/0.449 |
| Mean I/σI (overall/inner/outer shell) | 14.6/39.9/2.0 | 13.8/43.4/1.8 | 7.6/19.0/1.4 | 7.6/16.3/1.7 |
| Multiplicity (overall/inner/outer shell) | 7.2/7.0/7.3 | 6.8/6.6/6.6 | 4.8/4.7/4.9 | 3.1/2.9/3.1 |
| Number of reflections | 19,941 | 14,349 | 21,892 | 31,911 |
| Number of atoms | 1595 | 1515 | 3176 | 3924 |
| Waters | 190 | 114 | 54 | 65 |
| Rwork/Rfree (% data used) | 19.9/24.4 (5.1%) | 20.9/26.7 (5.0%) | 23.4/27.7 (5.0%) | 24.5/26.3 (5.05%) |
| rmsd from ideal values: bond length/angles | 0.011/1.478 | 0.009/1.310 | 0.015/1.619 | 0.007/0.900 |
| Mean B value | 26.52 | 31.553 | 59.69 | 70.80 |
| Correlation coefficient Fo-Fc/Fo-Fc free | 0.961/0.942 | 0.955/0.926 | 0.954/0.933 | 0.854/0.836 |
| Molprobity Score | 0.97 (100th percentile) | 1.2 (99th percentile) | 1.70 (96th percentile) | 1.40 (100th percentile) |