Figure 3.
Specificity of the rat antiSFFVgp52 Env and goat antiMLV Gag antibodies established by antibody depletion. Antibodies were pre-incubated (1 h, 25°C, constant shaking) with purified and heatinactivated retrovirus derived from 22Rv1, prior to probing duodenal biopsy sections. A: Duodenal biopsies were probed with polyclonal goat antibodies to MLV Gag. B: Duodenal biopsies were probed with polyclonal goat antibodies against MLV Gag preincubated with heatinactivated 22Rv1. C: Duodenal biopsies were probed with monoclonal rat antibody against SFFVgp52 Env. D: Duodenal biopsies were probed with monoclonal rat antibody against SFFVgp52 Env, preincubated with heat-inactivated 22Rv1 (Bar represents 20 µm).