Skip to main content
. Author manuscript; available in PMC: 2013 Sep 18.
Published in final edited form as: In Vivo. 2013 Mar-Apr;27(2):177–187.

Figure 4.

Figure 4

Evaluation of the specificity and potential crossreactivity of the antibodies used. A: Duodenal biopsies were probed with rhodomine-conjugated anti-SFFV-gp-52 Env IgG1κ monoclonal antibody (mAb) (1500). B: Duodenal biopsies were probed with rhodamine-conjugated donkey anti-rat IgG polyclonal antibody (pAb) (1:1000) and Dylight488-conjugated bovine anti-goat IgG pAb (1500). C: Duodenal biopsies were probed with rat anti-HHV8 ORF73 mAb (11,000), followed by incubation with rhodamine-conjugated donkey anti-rat IgG pAb (11000). D: Duodenal biopsies were probed with rat IgG1κ mAb isotype control (1:500), followed by incubation with rhodamine-conjugated donkey anti-rat IgG pAb (1:1,000) (Bar represents 20 µm). E: Duodenal biopsies were probed with normal goat serum (1:2,000), followed by incubation with Dylight488-conjugated bovine anti-goat IgG pAb (1:500). F: Duodenal biopsies were probed with rat anti-SFFV-gp-52 Env IgG1κ mAb, followed by incubation with Dylight488-conjugated bovine anti-goat IgG pAb (1:500). G: Duodenal biopsies were probed with goat anti-MLV-Gag IgG pAb followed by incubation with rhodamin-conjugated donkey anti-rat IgG pAb (1:1,000). TOPO3 was used to indicate nucleus localization in all images.