FIG. 3.

ALP activity of human and ovine BMSCs (a) and ADSCs (b) shown for each donor separately or as mean value from all combined hBMSCs or hADSCs (c). Donors marked with a prime symbol (′) indicates cells derived from the same donor but used in different round of experiments. Cells were cultured in control or osteogenic medium containing β-GP or NaH₂PO₄. ALP activity was measured by colorimetric assay at day 7 (D7) and 14 (D14). Each measurement was normalized for total DNA, as assayed by the Picogreen method. Values shown are means±SD. *p<0.05; **p<0.001, ***p<0.001, ****p<0.0001.