Cell viability and proliferation of hPLAP-tg BMSCs in culture for up to 7 days compared to wt BMSCs. (A) Cell viability assessed by MTS assay. For positive control, BMSCs were treated with latex rubber to induce cell death. (B) Cell proliferation assessed by DNA quantification. (C, D) Calcein-AM/propidium iodide live/dead staining of wt and hPLAP-tg BMSCs after 7 days of culture, P3. MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; Abs, absorbance.