FIG. 3.

Flow cytometric analysis of isolated and expanded wt and hPLAP-tg BMSCs. The cells were cultured for up to six passages and stained after every passage (from P2 to P6) for stem cell (CD29 and CD90) and hematopoietic markers (CD34 and CD45). (A) Density plot for FSC versus SSC and (B) percentage of cell viability of hPLAP-tg BMSCs at P4. (C) Expression of stem cell surface markers on hPLAP-tg and wt BMSCs at P4. Cells stained with the corresponding antibody are indicated by filled red histograms, negative isotype controls are indicated by open gray histograms.