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. 2013 Oct;2(5):346–355. doi: 10.1089/biores.2013.0027

FIG. 4.

FIG. 4.

Multiple lineage differentiation of wt and hPLAP-Tg BMSCs. (A) Osteogenic differentiation of wt and hPLAP-tg BMSCs (P2) cultured in osteogenic media for up to 21 days as indicated by alizarin red staining. (B) Adipogenic differentiation of wt and hPLAP-Tg BMSCs (P2) cultured in adipogenic media for up to 21 days as indicated by oil red O staining. (C) Chondrogenic differentiation of wt and hPLAP-tg BMSCs (P2) pellets cultured in chondrogenic media for up to 35 days as indicated by toluidine blue staining. Bar graphs show quantification of alizarin red–positive cultures (A), oil red O–positive cultures (B), and GAGs quantification (C) depending on culture time, passage (14 days of culture) and donor (cells in P2 after 14 days of culture).