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hPLAP expression of BMSCs isolated from wt and hPLAP-tg rats. (A) Histochemical hPLAP staining using NBT/BCIP after heat inactivation of hPLAP-tg BMSCs cultured for 24 h, 3 days, and 7 days after seeding. (B) Negative expression of the enzyme in wt BMSCs isolated and culture under identical conditions. (C) Higher magnification of hPLAP-tg BMSCs after 7 days of culture. NBT, nitrotetrazolium blue chloride; BCIP, 5-bromo-4-chloro-3-indolyl phosphate.
