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. 2013 Jun 4;21(9):1749–1757. doi: 10.1038/mt.2013.112

Figure 3.

Figure 3

Sunitinib inhibits eIF2α phosphorylation in response to viral infection of prostate tumors while increasing viral replication. Nude mice with s.c. PC3 prostate tumors were used. (a) Protein in tumors harvested 4 days postinfection were separated by SDS/PAGE, transferred, and probed with antibody to phosphorylated (Phos) eIF2α or Total eIF2α (as indicated). Lanes 1–5, untreated mice; lanes 6–10, mice received saline orally and tumors were infected with VSV; and lanes 11–15, mice received sunitinib orally and tumors were infected with VSV. (b) Quantitation of results in panel A determined by imageJ software. Data from two separate experiments are combined. **P < 0.01, ***P < 0.005. (c) Viral titers in extracts of tumors from mice determined by plaque assays. Data analysis was performed in GraphPadPrizm using Mann–Whitney test for statistical significance. Su, sunitinib; VSV, vesicular stomatitis virus.