Skip to main content
. 2013 Jun 4;21(9):1749–1757. doi: 10.1038/mt.2013.112

Figure 6.

Figure 6

Sunitinib inhibits phosphorylation of PKR and eIF2α in RCC ACHN cells infected with VSV (M51R) while increasing viral titers. (a) ACHN cells were incubated with or without sunitinib (5 µmol/l) for 2 hours and then mock infected or infected with VSV (M51R) at different multiplicity of infection (as indicated). The western blot was probed with antibodies as indicated. (b) VSV G protein and β-actin detection were by western blotting. (c) Viral titers by plaque assays were determined from ACHN cells incubated in the absence or presence of IFN-β (as indicated) for 3 hours followed by incubation in the absence or presence of sunitinib (5 µmol/l) for 2 hours (as indicated), and then infected with 1 multiplicity of infection VSV (M51R) for 13 hours in the continued presence of sunitinib where indicated. Data analysis was performed in GraphPadPrizm. **P < 0.01. IFN, interferon; MOI, multiplicity of infection; Su, sunitinib; VSV, vesicular stomatitis virus.