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. 2013 Jul 23;21(9):1695–1704. doi: 10.1038/mt.2013.143

Figure 3.

Figure 3

ZFN-Ad delivery in HaCaT cells. (a) HaCaT cells were infected with the indicated doses of ZFN-Ad (MOI) and donor-IDLV (ng p24) or Ad donor (MOI) and analyzed by fluorescence-activated cell sorting for GFP expression 3 and 12 days after transduction. (b) AAVS1-targeted PCR on genomic DNA isolated from HaCaT bulk populations 12 days after infection using primers specific for the 3′ integration junction amplifying a 1.7-kb band. (c) Cel-I assay on HaCaT cells infected with ZFN-Ad (MOI: 100, 200, and 500). The PCR product spanning the ZFN target site was loaded before and after nuclease treatment. Frequencies of gene disruption are indicated as % indels. Ad, adenoviral; IDLV, integration-defective lentiviral vector; MOI, multiplicity of infection; ZFN, zinc-finger nuclease.