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. 2013 Jul 9;21(9):1678–1686. doi: 10.1038/mt.2013.148

Figure 2.

Figure 2

Induction of p21CIP1/WAF1 by miR-34a in TP53-deficient cells. (a) Endogenous expression levels of target genes functioning in the miR-34a/TP53 axis were determined by quantitative reverse transcription-PCR (qRT-PCR) using RNA from SW48−/− and SW48+/+ cells transfected with either miR-34a or miR-215. Statistical significance of differential gene expression was determined by Student's t test (*P < 0.5; **P < 0.1; miRNA versus mock). (b) qRT-PCR results showing p21 mRNA levels in isogenic cell lines transfected with miR-34a. (c) Nonlinear regression analysis of p21 expression levels and proliferation rates in RKO−/− cells transfected with increasing concentrations of miR-34a ranging from 0.01 to 30 nmol/l. All values are normalized to those in mock-transfected cells (=1). Averages are shown. Standard deviations are included but are too small to be visible in the graph. *Statistical significance of p21 mRNA expression in miR-34a– versus miR-NC–transfected cells (Student's t test; P < 0.05). n, not detected.