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. 2013 Jul 9;21(9):1678–1686. doi: 10.1038/mt.2013.148

Figure 4.

Figure 4

Knock-down of HDAC1 mimics the miR-34a phenotype. (a) Knock-down of HDAC1 by small interfering RNA (siRNA) and miR-34a induces p21CIP1/WAF1 expression in TP53-negative cells. Protein lysates from cells transfected with miR-34a, miR-215, or si-HDAC1 were probed by Western blotting. Met was used as a positive control for miR-34a transfection; actin was used as a loading control. (b) Knock-down of HDAC1 by siRNA similarly inhibits cellular proliferation of SW48−/− and SW48+/+ cells. Cells were transfected with siRNAs and miRNAs, and cellular proliferation was measured 3 days thereafter. Values are normalized to mock (=100%). Averages and standard deviations are shown. P values are derived from two-tailed Student's t tests. The dotted line denotes the level cellular proliferation in cells transfected with miR-34a. HDAC1, histone deacetylase 1.